Журнал биоремедиации и биодеградации

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Biodegradation and Decolorization of Reactive Orange 16 by Nocardiopsis alba Soil Isolate

Sugumar Shobana and Berla Thangam

A bacterium identified as Nocardiopsis alba was isolated from acclimated sludge from a dyeing wastewater treatment plant. This strain rapidly decolorized Reactive orange16 (RO16) at 0.1% (w/v) concentration of both sucrose and peptone supplemented in Mineral Salt Medium (MSM) under static conditions at a temperature of 30°C with in 24 h with an initial dye concentration of 100 mg/L. The organism exhibited a remarkable color removal capability (95%) even at a high concentration of 1000 mg/L (RO16) dye within 24 h. The biodegradation products were analyzed by, FTIR spectroscopy and LC-MS analysis. The LC-MS analysis indicated the presence of 1-amino- 1-napthalene sulphonic acid in degraded product of the dye. The degraded product is less toxic to the growth of Vigna mungo seeds when compared to the non degraded dye.