Журнал аналитических и биоаналитических методов

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Chromatography Separating Molecules with Precision

Ying Wang

Chromatography, a fundamental analytical technique, has revolutionized the field of biochemistry by enabling the precise separation and identification of complex mixtures of molecules. This abstract provides an overview of chromatography’s significance, principles, and applications in various scientific domains. Chromatography operates on the principle of differential migration, where a sample mixture is partitioned between a stationary phase and a mobile phase. The components within the mixture travel at different rates through the stationary phase, leading to their separation based on factors such as size, charge, and affinity.