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Cloning and Analysis of N-Acetyltransferase 9 Genes in Yellow Catfish Pelteobagrus fulvidraco

Ming-Ming Han, Jian-Guo Lu, Le-Wang, Li-Na-Peng, Shahidd Mahboob, Khalid A Al-Ghanim and Xiao-Wen Sun

N-acetyltransferase 9 (NAT9) is an important reproduction-related gene in fish. In this study, we cloned the fulllength NAT9 cDNA sequence from yellow catfish Pelteobagrus fulvidraco. P. fulvidraco NAT9 cDNA is 1253 bp, which including non-coding region (584 bp) and open reading frame (669 bp). NAT9 gene encodes a 222 amino acidsprotein which shares high homology with NAT9 in four species: Ietalurus punetaus (91%), Astyanax mexicanus (83%), Danio rerio (81%) and Lepisosteus oculatus (81%). NAT9 protein is 25.7899 kD, 4.86 theoretical isoelectric point and C1124H1759N311O359S13 as revealed by computer-assisted analysis. Tissue transcription profile analysis indicated that the catfish NAT9 gene is generally but differentially expressed in the detected tissues, including gonads, brain, liver, kidney, spleen, intestine, blood, gills, muscle and heart. Identification and tissue distribution of yellow catfish NAT9 genes provided initial step towards understanding their biological roles in yellow catfish.