Биотехнологии и биоматериалы

Абстрактный

CoBRA-Containerized Bioinformatics in ChIP/ATAC-seq

Ava James*

Chromatin immunoprecipitation sequencing (ChIP-seq) and the Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) have grown to be crucial technology to successfully degree protein-DNA interactions and chromatin accessibility. However, there's a want for a scalable and reproducible pipeline that carries right normalization among samples, correction of reproduction quantity variations, and integration of recent downstream evaluation equipment. Containerized Bioinformatics workflow for Reproducible ChIP/ ATAC-seq Analysis (CoBRA), a modularized computational workflow which quantifies ChIP-seq and ATAC-seq height areas and plays unsupervised and supervised analyses [1]. CoBRA gives a complete modern-day ChIP-seq and ATAC-seq evaluation pipeline that may be utilized by scientists with confined computational revel in. This allows researchers to advantage speedy perception into protein-DNA interactions and chromatin accessibility thru pattern clustering, differential height calling, motif enrichment, assessment of web web sites to a reference database, and pathway evaluation.