Журнал аналитических и биоаналитических методов

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Differential Proteomic and Phospho-proteomic Analysis of Normal versus Failed Spermiation in Adult Rats by Label-Free LC-MS/MS

Rahul D Upadhyay, Amit Kumar Yadav, Shobha Sonawane, Reshma Goankar, Debasis Dash and Balasinor NH

Spermiation is the final step of spermatogenesis and involves release of mature spermatids from the Sertoli cells. It is a complex process involving displacement and removal of spermatid cytoplasm, formation and degradation of tubulobulbar complexes and progressive loss of adhesive junctions, including ectoplasmic specializations and subsequent phagocytosis of residual bodies by the Sertoli cell. Spermiation occurs in stage VIII of the rat seminiferous epithelium cycle. In spite of the important role played by spermiation process in sperm output to the epididymis, very little is known about this process. To enhance our knowledge of the sperm release biology, we sought to understand the molecular events occurring at the time of spermiation. Towards this aim, we induced spermiation failure condition by 17β estradiol treatment to adult male rats and compared with the normal spermiation. On comparing both the groups, we identified a total of 104 differentially expressed proteins and 23 differentially expressed phosphoproteins by LC-MS/MS analysis and quantitation. Localization and expression of some of the validated differential proteins in the testes highlight their importance during the sperm release. The present study represents an initial step to understand the molecular basis of the process of spermiation.