Журнал цитокиновой биологии

Абстрактный

Effects Of Low- And High-Frequency Repetitive Magnetic Stimulation on Vegetative Cell Proliferation and Protein Expression: A Preliminary Report

Masoumeh Mirmasoumi

Repetitive magnetic stimulation could be a medical specialty and neurorehabilitation tool that may be wont to investigate the biology of sensory and motor functions. Few studies have examined the results of repetitive magnetic stimulation on the modulation of neurotrophic/growth factors and somatic cell cells in vitro. Therefore, this study examined the differential effects of repetitive magnetic stimulation on somatic cell proliferation yet as numerous protein expressions. Immortalized mouse malignant tumor cells were used because the cell model during this study. Dishes of refined cells were every which way divided into management, sham, low-frequency (0.5 Hz, one Tesla) and high-frequency (10 cycles/second, one Tesla) teams (n = four dishes/group) and were stirred for three days. Expression of neurotropic/growth factors, Akt and Erk was investigated by Western blotting analysis three days when repetitive magnetic stimulation. Malignant tumor cell proliferation was resolute with a cell tally assay [1].There have been variations in cell proliferation supported information frequency. Low-frequency stimulation failed to alter proliferation relative to the management, whereas high-frequency stimulation elevated proliferation relative to the management cluster. The expression levels of brain-derived neurotrophic issue (BDNF), vegetative cell linederived neurotrophic issue (GDNF), neurotrophin-3 (NT-3) and platelet-derived protein (PDGF) was elevated within the high-frequency magnetic stimulation cluster. Akt and Erk expression was additionally considerably elevated within the high-frequency stimulation cluster, whereas low-frequency stimulation belittled the expression of Akt and Erk compared to the management. Last, we have a tendency to determine that completely different frequency magnetic stimulation had associate degree influence on somatic cell proliferation via regulation of Akt and ERK sign pathways and also the expression of growth factors like BDNF, GDNF, NT-3 and PDGF. These findings represent a promising chance to realize insight into however completely different frequencies of repetitive magnetic stimulation could mediate cell proliferation [2].