Журнал терапии ожирения и снижения веса

Абстрактный

Ghrelin Plasma Levels and Gastric Tissues Expression in Patients Submitted to Laparoscopic Sleeve Gastrectomy as Primary or Revisional Weight Loss Procedure

Claudio Di Cristofano, Caterina Chiappetta, Francesco De Angelis, Natale Porta, Jessica Cacciotti, Eugenio Lendaro, Vincenzo Petrozza, Carlo Della Rocca and Gianfranco Silecchia

Introduction: Ghrelin (Ghr) plays a role in the regulation of food intake and Laparoscopic Sleeve gastrectomy (LSG) is used for treatment of morbid obesity (MO) and after this the expression of ghrelin could be modulate. The aim of present study was to analyze the expression of ghr in three areas of resected stomach specimens from MO patients and co rrelate these data with plasmatic ghrelin levels before andafter surgery. Materials and Methods: 36 morbidly obese patients (17%, 6/36 with Type 2 Diabetes) were subjected to LSG and tissue samples were obtained from the fundus, body, prepyloric of the resected stomach. For mRNA and protein expression analysis. Blood samples were collected before and 1 month after surgery to evaluate the plasmatic ghrelin levels. Results: Ghrelin protein expression was higher in the fundus than in the other areas. T2DM patients showed a lower basal ghrelin plasma level compared with non-diabetic patients but they showed a high percentage of positive cells in the stomach. Was not observed a statistically difference in plasmatic, mRNA and protein expression of ghrelin between primary LSG patients and in revisional LSG group. Conclusion: Ghrelin fundal mucosal expression was comparable in primary and revisional LSG. Diabetic patients showed a compensatory higher protein mucosal expression probably to balance lower plasma Ghrelin level. Further studies will elucidate the clinical relevance of those preliminary data.