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Kuijiean Suppress Inflammation in Ulcerative Colitis Rat Models by Phosphorylation Level of HuR

Xilinguli Wushouer, Nafeisha Kadeer, Xu Chenbo, Kasimujiang Aximujiang, Zhang Jingping, Huang Jingjing and Kurexi Yunusi

In this study used a TNBS/ethanol method and combinated complex factors established the rat model of ulcerative colitis and treatment with the Xipayi Kuijiean (KJA) to conduct whole process. We detected the expression level of hnRNA and mRNA in the five inflammation factor such as IL-1α, IL-1β, IL-10, IL-17, TNF-α and regulatory protein NF-κB and HuR in UC with complex factor group (UC group), normal group, UC treated with KJA group (KJA group) and negative control group. In UC group, we assesed the colonic damage and colon length by naked eye decision scores, KJA significantly reduced the severity of colitis inflammation. The immune dysfunction tends to be basically restored in colon tissue of the KJA group. Furthermore, in UC group hnRNA and mRNA level are increased, but mRNA level significantly higher than hnRNA. The post-transcriptional level of inflammatory related factors was mainly followed by the reduction of mRNA level after treated with KJA. These results demonstrate that post-regulation level of HuR has an important role in the development of UC.