Журнал биоремедиации и биодеградации

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Microbial Decolorization and Degradation of Orange 16 Dye by a Newly Isolated Aeromonas Spp. Etl-1949

Maulin P Shah, Patel KA, Nair SS, Darji AM and Maharaul SJ

A bacterial strain ETL-1949, identified as Aeromonas spp. was isolated from sediment from a textile wastewater treatment plant in Ankleshwar, Gujarat, India. The strain could decolorize an azo dye, Orange 16 under the static condition after the shaking culture. In order to increase cell concentration by the shaking culture, the optimum culture condition was investigated for following the decolorization incubation. The dye was decolorized completely up to concentration of 750 mg/l for 50 hr under static condition by using 12 hr-cultured cells at optimal shaking culture condition. Effect of initial cell mass showed that higher cell mass concentration can accelerate decolorization process with maximum of 92% decolorization observed at 2.5 g/l cell mass within 6.5 h. Effect of various metal ions showed Mn & Mg has positive inducing effect whereas Zn strongly inhibited the decolorization process at 5 mM concentration. Analysis of biodegradation products carried out with UV–vis spectroscopy, HPTLC and FTIR confirmed the decolorization and degradation of Orange 16.