Журнал аналитических и биоаналитических методов

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PMMA Platform Based Micro Fluidic Mixer for the Detection of MicroRNA- 18a from Retinoblastoma Serum

Bindu Salim, Swapna Merlin David, Madhu Beta, Janakiraman Narayanan, Subramanian Krishnakumar and Thalakkotur Lazar Mathew

Retinoblastoma (RB) is an eye cancer found in children. Early diagnosis of RB is very crucial for the treatment to be effective. miRNA 18a is highly expressed in the serum of RB patients which can be used as a diagnostic marker to detect RB. A pillar based micro mixer is designed and fabricated on PMMA platform to hybridize the target miRNA 18a with its complementary quenched probe throughout the flow. The fluorescence can be measured in the monitoring well by the fluorescent reader at a particular wavelength and compared with the controls. The RB serums showed high fluorescence than the controls. The results were validated with the contemporary technique, Real-Time PCR and the results were concurring. Another advantage of the device is that the effort required for the immobilization of the probe on a platform is not needed, at the same time cost and time required for diagnosis is reduced.