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Maulin P Shah, Patel KA, Nair SS and Darji AM
Decolorization of azo dye by two bacterial species Bacillus megaterium and Bacillus cereus has been analyzed using mineral effluent, consisting of known concentration of the dye in ZZ medium. Physico chemical parameters such as carbon and nitrogen source, temperature, pH and inoculum volume are optimized for the decolorization process by changing one parameter at a time. Optimal condition for Bacillus cereus was found to be 1% sucrose, 0.25% peptone, pH 7, temperature 37°C and 8% inoculum and that for Bacillus megaterium was found to be glucose 1%, 0.25% yeast extract, pH 6, temperature 37°C and 10% inoculum. Extent of decolorization recorded by Bacillus cereus under ideal conditions was 95% and that by Bacillus megaterium was 98%. The study has confirmed the potential of Bacillus cereus and Bacillus megaterium in the decolorization of Azo dye and opened scope for future analysis of their performance in the treatment of textile effluent.