Журнал аналитических и биоаналитических методов

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Quantification of Hemoglobin Concentration in Whole Blood using Raman Spectroscopy

Fazal Fazlin, D. Matt Sublett, Arsenii Y Zhdanov, Keith M Bunch, Cori A Warren, Andrew A Blaurock, Hussain Chinoy, Victor G Samsonenko, Zohair Ghadiali

Rapid and accurate quantification of hemoglobin concentration in human blood samples is imperative to diagnose anemia. There are many methods that currently exist to do this, including manual and automatic laboratory methods and point-of-care devices. However, many of these methods necessitate the use of chemical reagents which can be expensive to purchase, store, and dispose of. Raman spectroscopy offers an alternative method of quickly and accurately determining hemoglobin concentration without the need for reagents. Here, we present a Raman based hemoglobin model that is valid from 1.7-24.8 g/dL. The method described here has a standard deviation of 1.3 g/dL and is shown to be precise within this standard deviation. This method was validated using 82 natural and artificial samples and demonstrates good agreement with current methods of hemoglobin quantification.