Журнал клинической и экспериментальной патологии

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Quantification of TUNEL Assay in Hippocampus of Diabetic Rats by MAT LAB: Comparison with Stereological Method

Shahriar Ahmadpour and Mohamad Amin Younessi Heravi

Quantification of immunostained sections is needed in order to reliably and accurately assesses the expression of cellular proteins in tissue. Here we describe a novel technique for quantification of TUNEL positive neurons in rat hippocampus. After uniform random sampling the slices were stained by TUNEL and counterstained by toluidine blue. Counting of TUNEL positive neurons performed by stereological method manually. The same sections were processed by MAT Lab software and the results compared together. The result showed there is no significant level of differences between the two methods (P<0.05). We believe merging image processing methods like MAT LAB and uniform random sampling facilitate the interpretation of study results.