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Using Rational Protein Engineering, Analyze the Expansion of Substrate and Template Specificities in DNA Strands

Sudha Vipin Sharma, Sunita Hooda

The use of enzymes in industrial applications has been recognised for providing clean processes with minimal impact on the environment. Enzyme engineering is undergoing the most profound and exciting transformation in its history. It promises unprecedented expansion in the scope and applications of modified or improved enzymes with desired physical and catalytic properties. Two complementary strategies are currently available: rational redesign and directed evolution. Although both approaches have met with great success, each has limitations. In this article,the perspectives for these enzyme-engineering strategies are discussed briefly.